HELICOBACTER PYLORI

In 1983, Warren and Marshall (1) identified Helicobacter pylori, a new gram-negative bacterial pathogen, in patients suffering from gastritis, and this finding led to studies on the relationship between bacterial infection and chronic gastric disease (2). The pathogen has been shown to be It has been demonstrated that in patients with gastritis, eradication of the bacteria led to healing of the anatomical lesion (4). Diagnostic procedures for the detection of the organism generally involve invasive (gastroscopic) techniques for sample collection. However, a specific immune response is seen in infected patients. The serological test thus represents a useful alternative to the invasive bioptic technique. IgG levels rise with infection and remain constantly high until the infection is eliminated. The efficacy of antimicrobial therapy can therefore be monitored via changes in specific IgG antibody. The determination of the IgA antibody levels is complementary to that of the IgG. As the IgA levels decrease more rapidly than the IgG in some patients undergoing treatment, this parameter can be useful in the follow-up of patients. For the same reason, a low IgA level in IgG-positive patients may indicate a past infection. For unknown reasons, about 2% of serum samples are positive only for IgA.

Method:

Immunoenzymatic method for the semiquantitative determination of IgG-class antibodies and qualitative determination of IgA-class antibodies to Helicobacter Pylori in human serum. The test is based on the ELISA principle (Enzyme Linked ImmunoSorbent Assay) which uses the reaction between the antibodies present in the tested sample and the immobilized antigen bound to solid phases. The immunoglobulins bind to the antigen through incubation with diluted human serum. The result is expressed as an INDEX (ratio between the OD value of the sample and that of the Cut-off) for qualitative tests and in International Units (UA/mL) for quantitative tests.

HELICOBACTER PYLORI IgA

coated with Helicobacter pylori

Kit for the determination of anti-Helicobacter pylori IgA antibodies in human serum
Code 91062
Quantity 96 tests
METHOD Indirect ELISA
TYPE Qualitative Test
DIAGNOSTIC SENSITIVITY 86.4%
DIAGNOSTIC SPECIFITY 96.7%
INCUBATION TIME 105 min
SHELF LIFE 15 months from the date of preparation
STORAGE and STABILITY 2°/8°C

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IFU

SDS

CoA

HELICOBACTER PYLORI IgG

coated with Helicobacter pylori antigens

Kit for the determination of anti-Helicobacter pylori IgG antibodies in human serum. Contains ready for use calibrators for the preparation of the calibration curve in Arbitrary Units (AU/mL).
Code 91060
Quantity 96 tests
METHOD Indirect ELISA
TYPE Semiquantitative test
DIAGNOSTIC SENSITIVITY 99.2%
DIAGNOSTIC SPECIFITY 90.9%
INCUBATION TIME 105 min
SHELF LIFE 15 months from the date of preparation
STORAGE and STABILITY 2°/8°C

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IFU

SDS

CoA

Bibliography

PRELIMINARY EVALUATION OF THE CHORUS SYSTEM FOR THE DETERMINATION OF IgG ANTI-HELICOBACTER PYLORI.