Adenoviruses are nonenveloped icosahedral particles of 60-90 nm in diameter with fiber proteins on each spike that facilitate the attack against the target cells: they represent the largest nonenveloped viruses. Because of their large size they are able to be transported inside the target cells through pores (envelope fusion is not necessary).The capsid is composed of 252 capsomeres of which 240 hexamers and 12 pentamers placed at the top; the apex of each vertex is a fiber which facilitates attachment to target cells resulting toxic, as is its base. The fiber also consists of viral proteins attack (VAP). Each adenovirus is constituted by a molecule of linear double-stranded DNA (dsDNA) formed by 30-36 Kb base pairs and coding for 30-40 genes, a number greater than viruses of the same cluster.Up to now, there are 100 serotypes of adenovirus , 57 of these have the potential to infect humans and are divided into six species. I their health effects range from respiratory illnesses to conjunctivitis gastroenteritis. The Adenovirus infections are common and frequent, and most of them are contracted in the childhood spell.
Method:
Immunoenzymatic method for the semiquantitative determination of complement-fixing antibodies in human serum, using a disposable device applied on the Chorus and Chorus TRIO instruments. The method uses antigens which are incubated for a given period with the test serum, in the presence of guinea pig complement. If specific antibodies are present, they will react with the antigen to form an immunocomplex which binds the complement, blocking its function. In the absence of specific antibodies the immunocomplex will not form and the free complement will cause lysis of the haemolytic mixture composed of sheep red blood cells and monoclonal hemolysin. The disposable devices contain all the reagents to perform the test when applied on the Chorus instruments.
