ANA-SCREEN

Method:

Immunoenzymatic method for the qualitative determination of IgG antibodies against HEp2 cells extract enriched with purified nuclear antigens (dsDNA, SS-B, SS-A 60/52 kDa, Scl-70, Cenp-B, Jo-1 snRNP complex (snRNP/Sm), Sm (screening) in human serum, using a disposable device applied on the Chorus and Chorus TRIO instruments.The test is based on the ELISA principle (Enzyme Linked ImmunoSorbent Assay) which uses the reaction between the antibodies present in the tested sample and the immobilized antigen bound to solid phases.The immunoglobulins bind to the antigen through incubation with diluted human serum.The disposable devices contain all the reagents to perform the test when applied on the Chorus instruments. The result is expressed as an INDEX (ratio between the OD value of the sample and that of the Cut-off).