CYTOMEGALOVIRUS

Cytomegalovirus is a herpes virus transmitted by close human contact. No symptoms of infection are apparent in the majority of cases. However, the virus is very dangerous and may be fatal in immunodepressed patients. Serum-negative female patients who become infected during pregnancy may transmit the disease to the fetus. In 95% of cases this occurs without symptoms, but some neonates may present jaundice, hepato-splenomegaly and retarded psycho-motorial development. For this reason it is of great importance to determine the immunitary state of the patient and check for serum conversion. A significant increase in the anti-Cytomegalovirus IgG titer is indicative of a recent infection or reactivation of a latent infection.Sometimes it is not sufficient to test for IgM antibodies, as the presence of this class may be due to the persistence of IgM or asymptomatic reinfection by Cytomegalovirus, without risk for the fetus. For this reason it is useful to assay the avidity of the IgG antibodies, in as far as it has been demonstrated that avidity is low in the acute phase of infection and increases over a period of time. The presence of low avidity antibodies is therefore an indication of a recent or current infection.

Method:

Immunoenzymatic method for the quantitative determination of IgG-class antibodies, qualitative determination of IgM-antibodies and the qualitative determination of the avidity of IgG class antibodies to Cytomegalovirus in human serum. The test is based on the ELISA principle (Enzyme Linked ImmunoSorbent Assay) which uses the reaction between the antibodies present in the tested sample and the immobilized antigen bound to solid phases. The immunoglobulins bind to the antigen through incubation with diluted human serum. The result is expressed as an INDEX (ratio between the OD value of the sample and that of the Cut-off) for qualitative tests and in International Units (IU/mL) for quantitative tests.

CMV SCREEN

coated with Cytomegalovirus antigens

Kit for the determination of anti-Cytomegalovirus immunoglobulins in human serum. Immunoenzymatic competitive method.
Code 91013
Quantity 96 tests
METHOD competition ELISA
TYPE Qualitative Test
DIAGNOSTIC SENSITIVITY 99,3%
DIAGNOSTIC SPECIFITY 99,8%
INCUBATION TIME 75 min
SHELF LIFE 15 months from the date of preparation
STORAGE and STABILITY 2°/8°C

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CoA

CYTOMEGALOVIRUS IgG

coated with Cytomegalovirus

Kit for the determination of anti-Cytomegalovirus IgG antibodies in human serum. Contains ready for use calibrators for the preparation of the calibration curve in International Units (IU/mL).
Code 91010
Quantity 96 tests
METHOD Indirect ELISA
TYPE Quantitative Test
DIAGNOSTIC SENSITIVITY 100%
DIAGNOSTIC SPECIFITY 100% CI95%: 97.1. – 100
INCUBATION TIME 105 min
SHELF LIFE 15 months from the date of preparation
STORAGE and STABILITY 2°/8°C

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CoA

CYTOMEGALOVIRUS IgG AVIDITY

coated with Cytomegalovirus

Kit for the determination of anti-Cytomegalovirus IgG antibodies avidity in human serum.
Code 91092
Quantity 24 tests
METHOD Indirect ELISA
TYPE Qualitative Test
INCUBATION TIME 120 min
SHELF LIFE 15 months from the date of preparation
STORAGE and STABILITY 2°/8°C

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CYTOMEGALOVIRUS IgM

coated with anti-human IgM monoclonal antibodies

Kit for the determination of anti-Cytomegalovirus IgM antibodies in human serum. Capture method.
Code 91011
Quantity 96 tests
METHOD CAPTURE ASSAY
TYPE Qualitative Test
DIAGNOSTIC SENSITIVITY 98.4 %
DIAGNOSTIC SPECIFITY 97.8 %
INCUBATION TIME 105 min
SHELF LIFE 15 months from the date of preparation
STORAGE and STABILITY 2°/8°C

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IFU

SDS

CoA

Bibliography

Analisi della concordanza analitica e clinica tra due sistemi analitici nella determinazione di anticorpi anti toxoplasma, rosoliavirus e cytomegalovirus